1SIE
MURINE POLYOMAVIRUS COMPLEXED WITH A DISIALYLATED OLIGOSACCHARIDE
Experimental procedure
| Spacegroup name | I 2 3 |
| 格子定数 [Å] | 570.000, 570.000, 570.000 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 12.000 - 3.650 |
| R因子 | 0.244 |
| Rwork | 0.244 |
| R-free | 0.26400 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 12.000 |
| 分解能 [Å] (高) | 3.650 |
| 独立反射数 | 244414 |
| 完全性 [%] | 74.0 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | virus | 6-8 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | sodium sulfate | 0.25-0.3 (M) | |
| 4 | 1 | drop | glycerol | 2.5-5 (%(v/v)) | |
| 5 | 1 | reservoir | sodium sulfate | 0.55-0.6 (M) | |
| 6 | 1 | reservoir | HEPES | 10 (mM) | |
| 7 | 1 | reservoir | glycerol | 5-10 (%) |
