1S32
Molecular Recognition of the Nucleosomal 'Supergroove'
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.352, 109.717, 181.827 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 2.050 |
Rwork | 0.219 |
R-free | 0.24340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aoi |
RMSD bond length | 0.008 |
RMSD bond angle | 1.099 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.0) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.076 | 0.353 |
Number of reflections | 124523 | |
<I/σ(I)> | 22.99 | 2.04 |
Completeness [%] | 94.4 | 54.1 |
Redundancy | 4.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | Manganese chloride, potassium chloride, potassium cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Manganese chloride | ||
2 | 1 | 1 | potassium chloride | ||
3 | 1 | 1 | potassium cacodylate | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | Manganese chloride | ||
6 | 1 | 2 | potassium chloride | ||
7 | 1 | 2 | H2O |