1S32
Molecular Recognition of the Nucleosomal 'Supergroove'
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 105.352, 109.717, 181.827 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 100.000 - 2.050 |
| Rwork | 0.219 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aoi |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.099 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 100.000 | 2.100 |
| 分解能 [Å] (高) | 2.050 | 2.050 |
| Rmerge_l_obs | 0.076 | 0.353 |
| 独立反射数 | 124523 | |
| <I/σ(I)> | 22.99 | 2.04 |
| 完全性 [%] | 94.4 | 54.1 |
| 冗長性 | 4.75 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | Manganese chloride, potassium chloride, potassium cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | Manganese chloride | ||
| 2 | 1 | 1 | potassium chloride | ||
| 3 | 1 | 1 | potassium cacodylate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Manganese chloride | ||
| 6 | 1 | 2 | potassium chloride | ||
| 7 | 1 | 2 | H2O |
