1S1Q
TSG101(UEV) domain in complex with Ubiquitin
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97979 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 143.634, 59.154, 93.998 |
| 格子定数 [度] | 90.00, 128.67, 90.00 |
精密化法
| 残基 | 72.550 - 2.000 |
| R因子 | 0.20319 |
| Rwork | 0.201 |
| R-free | 0.23998 |
| Structure solution method | SAD & Molecular replacement |
| Starting model (for MR) | 1KPP & 1UBO |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.019 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.357 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 72.550 | 2.100 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.335 | |
| 独立反射数 | 40921 | |
| <I/σ(I)> | 2.1 | |
| 完全性 [%] | 97.8 | 96.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 1.4M Ammonium sulfate, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
