1RVZ

1934 H1 Hemagglutinin in complex with LSTC

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-20
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
格子定数 [Å]	227.583, 131.522, 175.017
格子定数 [度]	90.00, 110.07, 90.00

精密化法

残基	20.000 - 2.250
R因子	0.225
Rwork	0.225
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ru7
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.000	2.330
分解能 [Å] (高)	2.250	2.250
Rmerge_l_obs	0.082	0.401
独立反射数	206016
<I/σ(I)>	14.6	2.9
完全性 [%]	89.9	52.2
冗長性	11.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	PEG3350, TrisHCl, KSCN, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K