1RKC
Human vinculin head (1-258) in complex with talin's vinculin binding site 3 (residues 1944-1969)
Experimental procedure
実験手法 | MIRAS |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Wavelength(s) | 0.9793,1.2545,0.9793 |
Spacegroup name | I 41 3 2 |
格子定数 [Å] | 155.680, 155.680, 155.680 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 15.000 - 2.700 |
Rwork | 0.244 |
R-free | 0.33500 |
Structure solution method | SAD |
結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
結合角の平均二乗偏差(RMSD) [度] | 1.263 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | SHARP |
Refinement software | BUSTER-TNT |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 45.000 * | |
分解能 [Å] (高) | 2.700 | 2.800 * |
Rmerge_l_obs | 0.100 * | 0.497 * |
Total number of observations | 178681 * | |
独立反射数 | 9051 | 1290 * |
完全性 [%] | 99.8 * | 99.7 * |
冗長性 | 19.6 * | 5.4 * |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion * | 4 | 293 | 2% MPD; 100 mM citric acid (pH 4); 100 mM CdCl2, pH 4.0, temperature 293K |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | reservoir | MPD | 2 (%) | |
2 | 1 | reservoir | citric acid | 100 (mM) | pH4 |
3 | 1 | reservoir | 100 (mM) |