1RKC

Human vinculin head (1-258) in complex with talin's vinculin binding site 3 (residues 1944-1969)

Experimental procedure

実験手法	MIRAS
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Detector technology	CCD
Wavelength(s)	0.9793,1.2545,0.9793
Spacegroup name	I 41 3 2
格子定数 [Å]	155.680, 155.680, 155.680
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	15.000 - 2.700
Rwork	0.244
R-free	0.33500
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.263
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SHARP
Refinement software	BUSTER-TNT

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	45.000 *
分解能 [Å] (高)	2.700	2.800 *
Rmerge_l_obs	0.100 *	0.497 *
Total number of observations	178681 *
独立反射数	9051	1290 *
完全性 [%]	99.8 *	99.7 *
冗長性	19.6 *	5.4 *

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion *	4	293	2% MPD; 100 mM citric acid (pH 4); 100 mM CdCl2, pH 4.0, temperature 293K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	reservoir	MPD	2 (%)
2	1	reservoir	citric acid	100 (mM)	pH4
3	1	reservoir		100 (mM)