1RH5

The structure of a protein conducting channel

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-01
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
格子定数 [Å]	102.930, 156.650, 69.800
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	10.000 - 3.200
R因子	0.242
Rwork	0.242
R-free	0.28700 *
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.230 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	10.000	3.310
分解能 [Å] (高)	3.200	3.200
Rmerge_l_obs	0.050 *	0.440 *
独立反射数	17396	1439 *
<I/σ(I)>	26.9	2.24
完全性 [%]	93.2	76.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	9.5	4 *	Peg 400, Glycine buffer, glycerol, sodium chloride, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir	PEG400	50-55 (%)
3	1	reservoir	glycine	50 (mM)	pH9.0-9.5