1RH5
The structure of a protein conducting channel
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 2 |
格子定数 [Å] | 102.930, 156.650, 69.800 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 10.000 - 3.200 |
R因子 | 0.242 |
Rwork | 0.242 |
R-free | 0.28700 * |
Structure solution method | SAD |
結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
結合角の平均二乗偏差(RMSD) [度] | 1.230 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 10.000 | 3.310 |
分解能 [Å] (高) | 3.200 | 3.200 |
Rmerge_l_obs | 0.050 * | 0.440 * |
独立反射数 | 17396 | 1439 * |
<I/σ(I)> | 26.9 | 2.24 |
完全性 [%] | 93.2 | 76.1 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 4 * | Peg 400, Glycine buffer, glycerol, sodium chloride, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | protein | 5-10 (mg/ml) | |
2 | 1 | reservoir | PEG400 | 50-55 (%) | |
3 | 1 | reservoir | glycine | 50 (mM) | pH9.0-9.5 |