1RF3
Structurally Distinct Recognition Motifs in Lymphotoxin-B Receptor and CD40 for TRAF-mediated Signaling
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 98 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 3 2 1 |
| 格子定数 [Å] | 83.700, 83.700, 79.100 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 8.000 - 3.500 |
| Rwork | 0.266 |
| R-free | 0.31500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1l0a |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.520 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 3.630 |
| 分解能 [Å] (高) | 3.500 | 3.500 |
| 独立反射数 | 3177 | |
| <I/σ(I)> | 8 | 8 |
| 完全性 [%] | 95.4 | 95 |
| 冗長性 | 4.43 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 15% PEG4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
