1R55

Crystal structure of the catalytic domain of human ADAM 33

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-17
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
格子定数 [Å]	57.990, 65.205, 99.679
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	25.000 - 1.580
R因子	0.21
Rwork	0.207
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	1.620
分解能 [Å] (高)	1.580	1.580
独立反射数	24596
完全性 [%]	93.5	56.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	10.5	277	PEG 800, sodium chloride, caps, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K