1QS1
CRYSTAL STRUCTURE OF VEGETATIVE INSECTICIDAL PROTEIN2 (VIP2)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 1997-12-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 53.900, 78.700, 105.900 |
| 格子定数 [度] | 108.65, 89.96, 90.02 |
精密化法
| 残基 | 20.000 - 1.500 |
| R因子 | 0.235 |
| Rwork | 0.235 |
| R-free | 0.26900 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 1.550 |
| 分解能 [Å] (高) | 1.500 | 1.500 |
| Rmerge_l_obs | 0.046 | 0.270 |
| Total number of observations | 468627 * | |
| 独立反射数 | 215237 | |
| <I/σ(I)> | 13 | |
| 完全性 [%] | 83.4 | 69.2 |
| 冗長性 | 2.2 | 1.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion * | 7.5 | 20 * | PEG200, BOG, IMIDAZOLE, MALATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | |
| 3 | 1 | reservoir | PEG200 | 30 (%(w/v)) | |
| 4 | 1 | reservoir | octyl-beta-D-glucopyranoside | 1 (%(w/v)) | |
| 5 | 1 | reservoir | imidazole/malate | 200 (mM) |
