1QI7
THE CRYSTAL STRUCTURE AT 2.0 A OF SAPORIN SO6, A RIBOSOME INACTIVATING PROTEIN FROM SAPONARIA OFFICINALIS
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 309 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 43 2 2 |
| 格子定数 [Å] | 67.530, 67.530, 119.670 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 15.000 - 2.000 |
| Rwork | 0.182 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1paf |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.240 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 15.000 | 2.230 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.109 * | 0.294 |
| Total number of observations | 210203 * | |
| 独立反射数 | 19206 | |
| 完全性 [%] | 98.5 | 96.7 |
| 冗長性 | 8.7 | 8.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 4.7 | 309 * | pH 4.7 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | reservoir solution | ||
| 3 | 1 | drop | PEG400 | 10 (%(w/v)) | |
| 4 | 1 | reservoir | ammonium sulfate | 2.8 (M) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) |
