1QH5

HUMAN GLYOXALASE II WITH S-(N-HYDROXY-N-BROMOPHENYLCARBAMOYL)GLUTATHIONE

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	1998-11-01
Detector	ADSC
Spacegroup name	P 21 21 21
格子定数 [Å]	39.550, 73.800, 164.700
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	20.000 - 1.450
R因子	0.204
Rwork	0.204
R-free	0.23200
Structure solution method	OTHER
結合長の平均二乗偏差(RMSD) [Å]	0.012
結合角の平均二乗偏差(RMSD) [度]	0.027
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	43.000	1.480
分解能 [Å] (高)	1.450	1.450
Rmerge_l_obs	0.115	0.340
Total number of observations	437671 *
独立反射数	84815 *
<I/σ(I)>	13	4
完全性 [%]	99.0	100
冗長性	5.2	3.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *	7.1 *		EQUILABRATION AGAINST 4-20% W/V PEG 2000 MONOMETHYL ETHER, pH 6.5

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	15 (mg/ml)
2	1	drop	MOPS	10 (mM)
3	1	drop	PEG400	25 (%)
4	1	reservoir	mPEG2000	4-20 (%)
5	1	reservoir	sodium imidazole	0.1 (M)
6	1	reservoir	dithiothreitol	2 (mM)