1QD7
PARTIAL MODEL FOR 30S RIBOSOMAL SUBUNIT
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-03-04 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.000, 1.700 |
| Spacegroup name | P 41 21 2 |
| 格子定数 [Å] | 401.600, 401.600, 174.500 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | ? - 5.500 |
| Structure solution method | MAD |
| Starting model (for MR) | PROTEINS WERE FIT INTO MAP AS RIGID BODIES FROM KNOWN CRYSTAL OR NMR STRUCTURES OF ISOLATED PROTEINS EXCEPT FOR S20 FOR WHICH NO HIGH RESOLUTION STRUCTURE EXISTS. S20 WAS FIT AS THREE-HELIX BUNDLE.S4 SEE REFERENCE (1); S5 MODELED ACCORDING TO PDB CODE 1PKP;S6 MODELED ACCORDING TO PDB CODE 1RIS;S7 MODELED ACCORDING TO PDB CODE 1RSS;S8 MODELED ACCORDING TO PDB CODE 1AN7;S15 MODELED ACCORDING TO PDB CODE 1A23;S17 SEE REFERENCE (7); |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (高) | 5.500 | 5.500 |
| 独立反射数 | 42000 | |
| <I/σ(I)> | 5.1 | |
| 完全性 [%] | 95.0 | 83 |
| 冗長性 | 5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 277 | Trakhanov, S.D., (1987) FEBS Lett., 220, 319. * |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 2 | MPD |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | 15 (%) |
