1PYI
CRYSTAL STRUCTURE OF A PPR1-DNA COMPLEX: DNA RECOGNITION BY PROTEINS CONTAINING A ZN2CYS6 BINUCLEAR CLUSTER
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1993-10-09 |
| Detector | XENTRONICS |
| Spacegroup name | I 2 2 2 |
| 格子定数 [Å] | 53.800, 92.600, 174.700 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 10.000 - 3.200 |
| R因子 | 0.245 |
| R-free | 0.33000 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.017 * |
| 結合角の平均二乗偏差(RMSD) [度] | 3.700 * |
| Data reduction software | XDS |
Quality characteristics
| Overall | |
| Rmerge_l_obs | 0.064 |
| 独立反射数 | 7096 |
| 冗長性 | 4.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 6.8 * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein dimer | 0.6 (mM) | |
| 10 | 1 | reservoir | MPD | 2 (%) | |
| 11 | 1 | reservoir | 125 (mM) | ||
| 12 | 1 | reservoir | zinc acetate | 0.100 (mM) | |
| 13 | 1 | reservoir | sodium cacodylate | 20 (mM) | pH6.8 |
| 2 | 1 | drop | DNA duplex | 0.7 (mM) | |
| 3 | 1 | drop | PEG3500 | 7 (%) | |
| 4 | 1 | drop | MPD | 1 (%) | |
| 5 | 1 | drop | 87.5 (mM) | ||
| 6 | 1 | drop | 62.5 (mM) | ||
| 7 | 1 | drop | zinc acetate | 0.050 (mM) | |
| 8 | 1 | drop | sodium cacodylate | 0.010 (mM) | pH6.8 |
| 9 | 1 | reservoir | PEG3500 | 15 (%) |
