1PYI
CRYSTAL STRUCTURE OF A PPR1-DNA COMPLEX: DNA RECOGNITION BY PROTEINS CONTAINING A ZN2CYS6 BINUCLEAR CLUSTER
Experimental procedure
Detector technology | AREA DETECTOR |
Collection date | 1993-10-09 |
Detector | XENTRONICS |
Spacegroup name | I 2 2 2 |
格子定数 [Å] | 53.800, 92.600, 174.700 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 10.000 - 3.200 |
R因子 | 0.245 |
R-free | 0.33000 |
結合長の平均二乗偏差(RMSD) [Å] | 0.017 * |
結合角の平均二乗偏差(RMSD) [度] | 3.700 * |
Data reduction software | XDS |
Quality characteristics
Overall | |
Rmerge_l_obs | 0.064 |
独立反射数 | 7096 |
冗長性 | 4.6 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion, hanging drop * | 6.8 * |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | protein dimer | 0.6 (mM) | |
10 | 1 | reservoir | MPD | 2 (%) | |
11 | 1 | reservoir | 125 (mM) | ||
12 | 1 | reservoir | zinc acetate | 0.100 (mM) | |
13 | 1 | reservoir | sodium cacodylate | 20 (mM) | pH6.8 |
2 | 1 | drop | DNA duplex | 0.7 (mM) | |
3 | 1 | drop | PEG3500 | 7 (%) | |
4 | 1 | drop | MPD | 1 (%) | |
5 | 1 | drop | 87.5 (mM) | ||
6 | 1 | drop | 62.5 (mM) | ||
7 | 1 | drop | zinc acetate | 0.050 (mM) | |
8 | 1 | drop | sodium cacodylate | 0.010 (mM) | pH6.8 |
9 | 1 | reservoir | PEG3500 | 15 (%) |