1PW2

APO STRUCTURE OF HUMAN CYCLIN-DEPENDENT KINASE 2

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-09-01
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
格子定数 [Å]	53.687, 71.799, 72.032
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	19.930 - 1.950
R因子	0.213
Rwork	0.213
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hcl
結合長の平均二乗偏差(RMSD) [Å]	0.005
結合角の平均二乗偏差(RMSD) [度]	22.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.000	2.000
分解能 [Å] (高)	1.950	1.950
Rmerge_l_obs	0.073	0.235 *
Total number of observations	222716 *
独立反射数	20576
<I/σ(I)>	16.5
完全性 [%]	97.1 *	98.4 *
冗長性	10.45

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.8	277	PEG 6000, Na-HEPES, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	7-8 (mg/ml)
2	1	reservoir	PEG6000	15 (%)
3	1	reservoir	sodium HEPES	100 (mM)	pH7.8-8.2