1ONJ

Crystal structure of Atratoxin-b from Chinese cobra venom of Naja atra

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRL BEAMLINE U7B
Synchrotron site	NSRL
Beamline	U7B
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2002-06-25
Detector	MARRESEARCH
Wavelength(s)	1.1516
Spacegroup name	P 41 21 2
格子定数 [Å]	49.110, 49.110, 44.580
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	16.570 * - 1.560 *
Rwork	0.171
R-free	0.19250
Structure solution method	MOLECULAR REPLACEMENT
Phasing software	AMoRE
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	16.570	1.640
分解能 [Å] (高)	1.560 *	1.560
Rmerge_l_obs	0.082 *	0.390 *
Total number of observations	87491 *
独立反射数	8305 *
完全性 [%]	99.9	99.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *	8.5	293	ammonium sulfate, Tris-HCl, pH 8.5, EVAPORATION, temperature 293K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	Tris-HCl	0.1 (M)	pH8.5
3	1	reservoir	ammonium sulfate	2.0 (M)