1OKE
Crystal structure of the dengue 2 virus envelope protein in complex with n-octyl-beta-D-glucoside
「1OAM」から置き換えられましたExperimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| 格子定数 [Å] | 81.602, 81.602, 287.408 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 45.000 - 2.400 |
| R因子 | 0.263 |
| Rwork | 0.263 |
| R-free | 0.29400 |
| Structure solution method | MIRAS |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.490 |
| 分解能 [Å] (高) | 2.400 | 2.400 |
| Rmerge_l_obs | 0.069 | 0.289 |
| 独立反射数 | 42998 | |
| <I/σ(I)> | 26.2 | 3.3 |
| 完全性 [%] | 96.8 | 74 |
| 冗長性 | 5.5 | 2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 8.5 | 11% PEG 8K, 1M NACL, 0.1M TRIS/HCL PH 9.0, 20% GLYCEROL,0.5% OCTYLGLUCOSIDE | ||
| 1 | 8.5 | 11% PEG 8K, 1M NACL, 0.1M TRIS/HCL PH 9.0, 20% GLYCEROL,0.5% OCTYLGLUCOSIDE |
