1NEZ

The Crystal Structure of a TL/CD8aa Complex at 2.1A resolution:Implications for Memory T cell Generation, Co-receptor Preference and Affinity

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	160
Wavelength(s)	1.01
Spacegroup name	P 31 2 1
格子定数 [Å]	77.018, 77.018, 176.045
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	50.000 - 2.100
Rwork	0.217
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.011 *
結合角の平均二乗偏差(RMSD) [度]	1.643 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000
分解能 [Å] (高)	2.100	2.100 *
Rmerge_l_obs	0.071	0.500 *
Total number of observations	171093 *
独立反射数	35619
<I/σ(I)>	9.7
完全性 [%]	98.9	100 *

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5	300	2.0M ammonium sulfate, 2.0% PEG 400, 5% DMSO, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	ammonium sulfate	2.0 (M)
3	1	reservoir	PEG400	2.0 (%)
4	1	reservoir	DMSO	5 (%)
5	1	reservoir	HEPES	0.1 (M)	pH7.5