1NEZ
The Crystal Structure of a TL/CD8aa Complex at 2.1A resolution:Implications for Memory T cell Generation, Co-receptor Preference and Affinity
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 160 |
Wavelength(s) | 1.01 |
Spacegroup name | P 31 2 1 |
格子定数 [Å] | 77.018, 77.018, 176.045 |
格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
残基 | 50.000 - 2.100 |
Rwork | 0.217 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
結合長の平均二乗偏差(RMSD) [Å] | 0.011 * |
結合角の平均二乗偏差(RMSD) [度] | 1.643 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 50.000 | |
分解能 [Å] (高) | 2.100 | 2.100 * |
Rmerge_l_obs | 0.071 | 0.500 * |
Total number of observations | 171093 * | |
独立反射数 | 35619 | |
<I/σ(I)> | 9.7 | |
完全性 [%] | 98.9 | 100 * |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | 2.0M ammonium sulfate, 2.0% PEG 400, 5% DMSO, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
3 | 1 | reservoir | PEG400 | 2.0 (%) | |
4 | 1 | reservoir | DMSO | 5 (%) | |
5 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |