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1N32

Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer RNA anticodon stem-loop mismatched at the first codon position at the a site with paromomycin

Experimental procedure
実験手法SINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID14-4
Synchrotron siteESRF
BeamlineID14-4
Temperature [K]90
Detector technologyCCD
Collection date2001-05-05
DetectorADSC QUANTUM 4
Spacegroup nameP 41 21 2
格子定数 [Å]400.296, 400.296, 175.483
格子定数 [度]90.00, 90.00, 90.00
精密化法
残基141.420 - 3.000
R因子0.227
Rwork0.227
R-free0.27000
Structure solution methodDIFFERENCE FOURIER
Starting model (for MR)1J5E WITHOUT IONS AND PORTIONS AROUND THE SITE
結合長の平均二乗偏差(RMSD) [Å]0.006
結合角の平均二乗偏差(RMSD) [度]1.200

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Quality characteristics
 OverallOuter shell
分解能 [Å] (低)500.000

*

3.110
分解能 [Å] (高)3.000

*

3.000
Rmerge_l_obs0.128

*

0.564

*

Total number of observations918025

*

独立反射数272610
<I/σ(I)>72
完全性 [%]95.580.1
冗長性3.522.91
結晶化条件
結晶ID方法pH温度溶液条件
1VAPOR DIFFUSION, HANGING DROP6.5277Clemons, W.M., (2001) J. Mol. Biol., 310, 827.

*

結晶化試薬
ID結晶ID濃度試薬名濃度 (単位)詳細
111NH4Cl
1012CaCl2
1112magnesium acetate
1212MPD
211KCl
311CaCl2
411magnesium acetate
511sodium cacodylate
611potassium-MES
711MPD
812NH4Cl
912KCl
文献の結晶化試薬
ID結晶ID溶液試薬名濃度 (単位) (単位)詳細
11reservoirMPD14-16 (%(v/v))
21reservoir0.2 (M)
31reservoir75 (mM)
41reservoir15 (mM)
51reservoirMES0.1 (M)pH6.5

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件を2024-09-18に公開中

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