1N32
Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer RNA anticodon stem-loop mismatched at the first codon position at the a site with paromomycin
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-05-05 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 41 21 2 |
| 格子定数 [Å] | 400.296, 400.296, 175.483 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 141.420 - 3.000 |
| R因子 | 0.227 |
| Rwork | 0.227 |
| R-free | 0.27000 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1J5E WITHOUT IONS AND PORTIONS AROUND THE SITE |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 500.000 * | 3.110 |
| 分解能 [Å] (高) | 3.000 * | 3.000 |
| Rmerge_l_obs | 0.128 * | 0.564 * |
| Total number of observations | 918025 * | |
| 独立反射数 | 272610 | |
| <I/σ(I)> | 7 | 2 |
| 完全性 [%] | 95.5 | 80.1 |
| 冗長性 | 3.52 | 2.91 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | Clemons, W.M., (2001) J. Mol. Biol., 310, 827. * |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | NH4Cl | ||
| 10 | 1 | 2 | CaCl2 | ||
| 11 | 1 | 2 | magnesium acetate | ||
| 12 | 1 | 2 | MPD | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | CaCl2 | ||
| 4 | 1 | 1 | magnesium acetate | ||
| 5 | 1 | 1 | sodium cacodylate | ||
| 6 | 1 | 1 | potassium-MES | ||
| 7 | 1 | 1 | MPD | ||
| 8 | 1 | 2 | NH4Cl | ||
| 9 | 1 | 2 | KCl |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | MPD | 14-16 (%(v/v)) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | 75 (mM) | ||
| 4 | 1 | reservoir | 15 (mM) | ||
| 5 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
