1MTB
Viability of a drug-resistant HIV-1 protease mutant: structural insights for better antiviral therapy
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 51.439, 60.039, 62.021 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 26.560 - 2.500 |
| R因子 | 0.191 |
| Rwork | 0.191 |
| R-free | 0.25700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1f7a |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 26.500 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 26.600 | 2.590 |
| 分解能 [Å] (高) | 2.500 | 2.500 |
| Rmerge_l_obs | 0.080 * | 0.360 * |
| Total number of observations | 28072 * | |
| 独立反射数 | 6743 | 496 * |
| <I/σ(I)> | 7 | |
| 完全性 [%] | 93.4 | 71 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | sodium phosphate, sodium citrate, ammonium sulphate, pH 6.2, VAPOR DIFFUSION, HANGING DROP at 298K |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | reservoir | phosphate | 126 (mM) | pH6.2 |
| 3 | 1 | reservoir | sodium citrate | 63 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 28-31 (%) |
