1M9C
X-ray crystal structure of Cyclophilin A/HIV-1 CA N-terminal domain (1-146) M-type Complex.
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-07-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 38.445, 113.235, 67.006 |
| 格子定数 [度] | 90.00, 100.53, 90.00 |
精密化法
| 残基 | 38.000 * - 2.000 |
| R因子 | 0.201 |
| Rwork | 0.195 |
| R-free | 0.25900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ak4 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.018 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.0) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 38.000 | 2.030 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.063 | 0.195 |
| Total number of observations | 89358 * | |
| 独立反射数 | 31588 | |
| <I/σ(I)> | 14.8 | 2.8 |
| 完全性 [%] | 83.0 | 60 |
| 冗長性 | 2.8 | 1.55 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 21 * | PEG 8K, Bicine, LiCl, Tris, Beta-mercaptoethanol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | CypA-CAN | 0.4 (mM) | |
| 2 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 3 | 1 | drop | Tris | 10 (mM) | pH8.0 |
| 4 | 1 | reservoir | PEG8000 | 15-25 (%(w/v)) | |
| 5 | 1 | reservoir | 1 (M) | ||
| 6 | 1 | reservoir | Bicine | 100 (mM) | pH7.0-9.0 |
