1M1G
Crystal Structure of Aquifex aeolicus N-utilization substance G (NusG), Space Group P2(1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.500, 54.400, 113.200 |
| Unit cell angles | 90.00, 89.30, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.510 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 * | 0.406 * |
| Number of reflections | 69671 | |
| <I/σ(I)> | 8.9 | 1.6 |
| Completeness [%] | 94.9 | 79.2 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 18 * | PEG 3000, sodium chloride, pH 8.2, Vapor diffusion, sitting drop, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES/Tris | 0.1 (M) | pH8.2 |
| 2 | 1 | reservoir | PEG3000 | 15 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | drop | Tris-HCl | 20 (mM) | pH7.0 |
| 5 | 1 | drop | 50 (mM) | ||
| 6 | 1 | drop | DNA | 1 (M) |
