1LPL
Structural Genomics of Caenorhabditis elegans: CAP-Gly domain of F53F4.3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.74 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 64.156, 64.156, 101.946 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 * - 1.770 |
R-factor | 0.225 * |
Rwork | 0.220 |
R-free | 0.29400 * |
Structure solution method | SAS |
RMSD bond length | 0.021 * |
RMSD bond angle | 1.700 * |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | ISAS (V. 2001) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.043 * | 0.369 * |
Number of reflections | 12651 * | |
Completeness [%] | 99.2 * | 95 * |
Redundancy | 9.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.4 * | 298 | Reservoir solution contained 1.8 M ammonium sulphate and 0.1 M MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
2 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
3 | 1 | drop | protein | 10 (mg/ml) | |
4 | 1 | drop | HEPES | 20 (mM) | pH7.4 |