1LP3
The Atomic Structure of Adeno-Associated Virus (AAV-2), a Vector for Human Gene Therapy
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-04-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 |
格子定数 [Å] | 249.690, 249.690, 644.760 |
格子定数 [度] | 90.00, 101.16, 120.00 |
精密化法
残基 | 39.800 - 3.000 |
R因子 | 0.338 |
Rwork | 0.338 |
R-free | 0.34200 |
Structure solution method | Molecular replacement at 15A, then phase extension by symmetry averaging |
Starting model (for MR) | PDB entries 4dpv 1dnv |
結合長の平均二乗偏差(RMSD) [Å] | 0.020 |
結合角の平均二乗偏差(RMSD) [度] | 26.680 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | CNS |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 100.000 * | 3.560 |
分解能 [Å] (高) | 3.000 | 3.000 * |
Rmerge_l_obs | 0.156 | 0.309 |
Total number of observations | 747988 * | |
独立反射数 | 495356 * | |
<I/σ(I)> | 6.3 | 5.9 |
完全性 [%] | 56.0 | 80 * |
冗長性 | 2 | 2 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion * | 7.3 | 295 | PEG 6000, magnesium chloride, hepes, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | protein | 8.5 (mg/ml) | |
2 | 1 | drop | glycerol | 25 (%) | |
3 | 1 | reservoir | PEG6000 | 4-5 (%) |