1KU7
Crystal Structure of Thermus aquatics RNA Polymerase SigmaA Subunit Region 4 Bound to-35 Element DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2001-08-09 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.180, 62.650, 94.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.400 |
R-factor | 0.255 |
Rwork | 0.255 |
R-free | 0.29300 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.052 * | 0.197 * |
Total number of observations | 29108 * | |
Number of reflections | 7801 * | |
<I/σ(I)> | 2.9 | |
Completeness [%] | 97.7 | 97.7 * |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 * | 22.5 * | MES, PEG 4000, magnesium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MES | ||
2 | 1 | 1 | PEG 4000 | ||
3 | 1 | 1 | magnesium acetate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
3 | 1 | reservoir | ammonium sulfate | 2.4 (M) | |
4 | 1 | reservoir | 20 (mM) |