1JZ6
E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH GALACTO-TETRAZOLE
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-03-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 149.690, 168.400, 200.920 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 17.000 - 2.100 |
| R因子 | 0.157 * |
| Rwork | 0.163 |
| R-free | 0.26800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dp0 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.017 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.800 * |
| Data reduction software | SDMS |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | TNT |
| Refinement software | TNT |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 17.000 | |
| 分解能 [Å] (高) | 2.100 | 2.100 |
| Rmerge_l_obs | 0.088 | 0.380 |
| 独立反射数 | 248629 | |
| <I/σ(I)> | 12.4 | 2.7 |
| 完全性 [%] | 86.0 * | 87.2 |
| 冗長性 | 4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion * | 6.5 | 288 | used macroseeding, Juers, D.H., (2000) Protein Sci., 9, 1685. * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 10 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 2 | 1 | drop | Bis-Tris | 100 (mM) | pH6.5 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | reservoir | PEG8000 | 10 (%) | |
| 7 | 1 | reservoir | bis-Tris | 100 (mM) | pH6.5 |
| 8 | 1 | reservoir | 200 (mM) | ||
| 9 | 1 | reservoir | 100 (mM) |
