1IKA

STRUCTURE OF ISOCITRATE DEHYDROGENASE WITH ALPHA-KETOGLUTARATE AT 2.7 ANGSTROMS RESOLUTION: CONFORMATIONAL CHANGES INDUCED BY DECARBOXYLATION OF ISOCITRATE

Experimental procedure

Spacegroup name	P 43 21 2
格子定数 [Å]	105.100, 105.100, 150.300
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	30.000 - 2.700
R因子	0.185
Rwork	0.185
結合長の平均二乗偏差(RMSD) [Å]	0.017
結合角の平均二乗偏差(RMSD) [度]	3.700
Phasing software	X-PLOR
Refinement software	PROLSQ

Quality characteristics

	Overall
分解能 [Å] (高)	2.700 *
Rmerge_l_obs	0.108 *
独立反射数	14729 *

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	20 (mg/ml)
2	1	drop	ammonium sulfate	40 (%sat)
3	1	reservoir	ammonium sulfate