1IDC
ISOCITRATE DEHYDROGENASE FROM E.COLI (MUTANT K230M), STEADY-STATE INTERMEDIATE COMPLEX DETERMINED BY LAUE CRYSTALLOGRAPHY
Experimental procedure
実験手法 | LAUE |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Detector technology | IMAGE PLATE |
Collection date | 1993-10 |
Detector | FUJI |
Wavelength(s) | 0.7 - 2.1 |
Spacegroup name | P 43 21 2 |
格子定数 [Å] | 105.100, 105.100, 150.300 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 10.000 * - 2.500 |
R因子 | 0.169 |
Rwork | 0.169 |
結合長の平均二乗偏差(RMSD) [Å] | 0.018 |
結合角の平均二乗偏差(RMSD) [度] | 25.070 * |
Data reduction software | OXFORD (LAUE PACKAGE (J.CAMPBELL)) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 100.000 * | |
分解能 [Å] (高) | 2.500 * | 2.500 * |
Rmerge_l_obs | 0.089 | |
独立反射数 | 17316 | |
完全性 [%] | 62.0 | 82 * |
冗長性 | 18.7 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | unknown * | 5.4 * |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | reservoir | dephosphorylated IDH | 28 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 34 (%sat) | |
3 | 1 | reservoir | 100 (mM) | ||
4 | 1 | reservoir | 35 (mM) | ||
5 | 1 | reservoir | citric acid | 9 (mM) | |
6 | 1 | reservoir | dithiothreitol | 0.2 (mM) |