1I9A
STRUCTURAL STUDIES OF CHOLESTEROL BIOSYNTHESIS: MEVALONATE 5-DIPHOSPHATE DECARBOXYLASE AND ISOPENTENYL DIPHOSPHATE ISOMERASE
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2000-12-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97940, 0.97900 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 72.758, 72.758, 204.471 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.27900 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.390 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.070 | 0.308 |
Number of reflections | 34202 | |
<I/σ(I)> | 18.2 | 3.5 |
Completeness [%] | 96.2 | 91.1 |
Redundancy | 3.7 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 8% PEG 8K, 15% PEG 1K 100mM NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |