1I5L
CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS COMPLEXED WITH SHORT POLY-U RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID13 |
Synchrotron site | ESRF |
Beamline | ID13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.964 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.858, 130.438, 70.047 |
Unit cell angles | 90.00, 115.36, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.750 |
R-factor | 0.225 * |
Rwork | 0.225 |
R-free | 0.30000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i4k |
RMSD bond length | 0.008 |
RMSD bond angle | 1.600 |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.050 | 0.354 |
Total number of observations | 70211 * | |
Number of reflections | 28746 | |
<I/σ(I)> | 11.2 | 2 |
Completeness [%] | 97.6 | 97.5 |
Redundancy | 2.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.3 * | 20 * | PEG6000, sodium citrate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG6000 | ||
2 | 1 | 1 | sodium citrate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 12 (%) | |
3 | 1 | reservoir | sodium citrate | 100 (mM) | pH4.3 |