1HR0
CRYSTAL STRUCTURE OF INITIATION FACTOR IF1 BOUND TO THE 30S RIBOSOMAL SUBUNIT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-07-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 399.581, 399.581, 176.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 99.000 * - 3.200 |
R-factor | 0.218 * |
Rwork | 0.218 |
R-free | 0.26090 |
Structure solution method | Difference Fourier using 30S as starting model |
Starting model (for MR) | 1FJF |
RMSD bond length | 0.007 |
RMSD bond angle | 28.450 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.800 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.140 | 0.499 |
Total number of observations | 854384 * | |
Number of reflections | 227537 * | |
<I/σ(I)> | 5.1 | 1.92 |
Completeness [%] | 96.2 | 92.2 |
Redundancy | 3.75 | 3.12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.5 | 277 | Wimberly, B.T., (2000) Nature, 407, 327. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NH4Cl | ||
10 | 1 | 2 | MPD | ||
11 | 1 | 3 | initiation factor 1 | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | magnesium acetate | ||
4 | 1 | 1 | potasium cacodylate | ||
5 | 1 | 1 | MPD | ||
6 | 1 | 2 | NH4Cl | ||
7 | 1 | 2 | KCl | ||
8 | 1 | 2 | magnesium acetate | ||
9 | 1 | 2 | potasium cacodylate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
4 | 1 | 2-mercaptoethanol | 6 (mM) | ||
5 | 1 | potassium cacodylate | 0.1 (M ) | or 0.1M MES | |
6 | 1 | 1 | MPD | 13-17 (%) |