1H65
Crystal structure of pea Toc34 - a novel GTPase of the chloroplast protein translocon
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1998-06-15 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9796, 0.9794, 0.9680, 0.9802 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 143.160, 78.673, 67.279 |
| 格子定数 [度] | 90.00, 91.44, 90.00 |
精密化法
| 残基 | 31.000 * - 2.000 |
| R因子 | 0.186 |
| Rwork | 0.186 |
| R-free | 0.23000 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 21.900 * |
| Data reduction software | DPS |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 31.000 | 2.100 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.056 | 0.223 |
| 独立反射数 | 50147 | |
| <I/σ(I)> | 8 | 1.9 |
| 完全性 [%] | 99.0 | 98.8 |
| 冗長性 | 7 | 2.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | THE TOC34 CRYSTALS WERE GROWN AT 4 DEGREES CELSIUS BY HANGING DROP VAPOR DIFFUSION. A 2-MICROLITER SOLUTION (10 MG/ML IN 50 MM TRIS-HCL AT PH 8.0 AND 0.1 M NACL) WAS MIXED WITH A 2-MICROLITER RESERVOIR SOLUTION CONTAINING 22% PEGMME 5K AND 10% GLYCEROL IN 0.1 M HEPES AT PH 6.5. |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH8.0 |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | reservoir | PEG5000 MME | 22 (%(w/v)) | |
| 5 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) | pH6.5 |
