1GIH
HUMAN CYCLIN DEPENDENT KINASE 2 COMPLEXED WITH THE CDK4 INHIBITOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 294 |
Detector technology | IMAGE PLATE |
Collection date | 1998-12-07 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.870, 72.670, 53.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.800 |
R-factor | 0.169 * |
Rwork | 0.169 |
R-free | 0.27500 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.677 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | AMoRE |
Refinement software | X-PLOR (98.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 * | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.075 | 0.075 |
Total number of observations | 38408 * | |
Number of reflections | 7545 | |
<I/σ(I)> | 9 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.1 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 * | 4 * | HEPES, sodium chloride, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | HEPES-NaOH | 10 (mM) | pH7.4 |
3 | 1 | drop | 15 (mM) | ||
4 | 1 | reservoir | HEPES-NaOH | 800 (mM) | pH7.4 |