1F3M
CRYSTAL STRUCTURE OF HUMAN SERINE/THREONINE KINASE PAK1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 1999-08-15 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 41 |
Unit cell lengths | 94.583, 94.583, 147.497 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.080 - 2.300 |
R-factor | 0.237 * |
Rwork | 0.237 |
R-free | 0.25800 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.320 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.080 | 2.370 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.069 | 0.349 |
Total number of observations | 160255 * | |
Number of reflections | 56156 | |
<I/σ(I)> | 15.4 | |
Completeness [%] | 97.9 | 97.4 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 12 * | Ammonium Sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.8-1.9 (M) | |
2 | 1 | reservoir | 150 (mM) | ||
3 | 1 | reservoir | 250-300 (mM) | ||
4 | 1 | reservoir | dithiothreitol | 10 (mM) | |
5 | 1 | reservoir | PIPES | 100 (mM) | |
6 | 1 | drop | 125 (mM) | ||
7 | 1 | drop | Tris-HCl | 20 (mM) | |
8 | 1 | drop | protein | 15-20mg |