1EO7
BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE IN COMPLEX WITH MALTOHEXAOSE
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 111.648, 109.022, 64.677 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 7.000 - 2.480 |
| R因子 | 0.228 * |
| Rwork | 0.228 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cxl |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.719 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 78.000 | 2.550 |
| 分解能 [Å] (高) | 2.480 | 2.490 |
| Rmerge_l_obs | 0.061 | 0.225 |
| 独立反射数 | 26146 | |
| <I/σ(I)> | 14.36 | 4.2 |
| 完全性 [%] | 91.2 | 86.4 |
| 冗長性 | 3.27 | 2.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion * | 8.1 * | 293 | Lawson, C.L., (1994) J. Mol. Biol., 236, 590. * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | MPD | 60 (%(v/v)) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 3 | 1 | reservoir | maltose | 5 (%(w/v)) |
