1EBD
DIHYDROLIPOAMIDE DEHYDROGENASE COMPLEXED WITH THE BINDING DOMAIN OF THE DIHYDROLIPOAMIDE ACETYLASE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1995 |
| Detector | RIGAKU |
| Spacegroup name | P 31 2 1 |
| 格子定数 [Å] | 106.600, 106.600, 204.300 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 8.000 - 2.600 |
| R因子 | 0.215 |
| Rwork | 0.215 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 24.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (高) | 2.600 * | 2.640 * |
| Rmerge_l_obs | 0.092 * | |
| 独立反射数 | 44969 | |
| 完全性 [%] | 95.0 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion * | 4.9 * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 9-10 (mg/ml) | |
| 2 | 1 | reservoir | PEG600 | 12 (%) | |
| 3 | 1 | reservoir | zinc acetate | 200 (mM) | |
| 4 | 1 | reservoir | FAD | 0.001 (mM) |
