1A8V
STRUCTURE OF THE RNA-BINDING DOMAIN OF THE RHO TRANSCRIPTION TERMINATOR
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 30.420, 36.160, 54.250 |
| 格子定数 [度] | 99.06, 102.64, 96.43 |
精密化法
| 残基 | 20.000 - 2.000 |
| R因子 | 0.193 * |
| Rwork | 0.193 |
| R-free | 0.26100 |
| Structure solution method | MIR |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 * |
| 結合角の平均二乗偏差(RMSD) [度] | 2.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 2.070 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.068 | 0.169 |
| 独立反射数 | 14432 | |
| <I/σ(I)> | 18.9 | 7 |
| 完全性 [%] | 98.9 | 95.8 |
| 冗長性 | 4.5 | 2.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 30 * | pH 8.5 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 10-20 (%) | |
| 3 | 1 | reservoir | ammonium acetate | 50-100 (mM) | |
| 4 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
| 5 | 1 | reservoir | 4 (mM) | ||
| 6 | 1 | reservoir | erbium acetate | 2 (mM) |
