1A1X
CRYSTAL STRUCTURE OF MTCP-1 INVOLVED IN T CELL MALIGNANCIES
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09 |
| Detector | RIGAKU |
| Spacegroup name | P 62 2 2 |
| 格子定数 [Å] | 62.665, 62.665, 85.962 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 6.500 - 2.000 |
| R因子 | 0.211 |
| Rwork | 0.211 |
| R-free | 0.25300 |
| Structure solution method | MAD PHASING |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 26.920 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MADSYS |
| Refinement software | X-PLOR (3.8) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 21.000 | 2.070 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.047 * | 0.147 * |
| 独立反射数 | 7194 | |
| <I/σ(I)> | 15.1 | 7.6 |
| 完全性 [%] | 99.7 | 99.3 |
| 冗長性 | 5.9 | 5.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion * | 7.8 | PROTEIN WAS CRYSTALLIZED FROM 1.5M AMS WITH TRIZMA BUFFER AT PH 7.8 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 5.0 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 3 | 1 | reservoir | 2-mercaptoethanol | 1.0 (mM) |
