1ZZK
Crystal Structure of the third KH domain of hnRNP K at 0.95A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 51.848, 30.876, 42.413 |
| Unit cell angles | 90.00, 102.74, 90.00 |
Refinement procedure
| Resolution | 40.000 - 0.950 |
| R-factor | 0.124 |
| Rwork | 0.119 |
| R-free | 0.12700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.037 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.000 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.057 | 0.170 |
| Number of reflections | 40192 | |
| <I/σ(I)> | 16.4 | 5 |
| Completeness [%] | 97.4 | 91.8 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | ammonium sulphate, PEG 400, Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
