1ZZB

Crystal Structure of CoII HppE in Complex with Substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	100
Spacegroup name	P 65 2 2
Unit cell lengths	86.240, 86.240, 220.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.120 - 2.300
R-factor	0.238
Rwork	0.238
R-free	0.28100
Structure solution method	RIGID BODY REFINEMENT
Starting model (for MR)	1zzc
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.120	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	22149
<I/σ(I)>	26.4	6.4
Completeness [%]	99.3	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		pH 8.5