1ZYZ
Structures of Yeast Ribonucloetide Reductase I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 433.696, 433.696, 433.696 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.21934 |
| Rwork | 0.217 |
| R-free | 0.25725 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | native structure |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.585 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0007) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.088 | 0.476 |
| Number of reflections | 73347 | |
| <I/σ(I)> | 30.2 | 2.7 |
| Completeness [%] | 95.0 | 93.8 |
| Redundancy | 11.1 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 298 | sodium acetate, sodium formate, pH 4.6, EVAPORATION, temperature 298K |
