1ZYO
Crystal Structure of the Serine Protease Domain of Sesbania Mosaic Virus polyprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.072, 74.072, 68.753 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.25234 |
| Rwork | 0.250 |
| R-free | 0.28821 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.211 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0009) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.055 | 0.266 |
| Number of reflections | 9272 | |
| <I/σ(I)> | 32.13 | 3.8 |
| Completeness [%] | 92.7 | 56.5 |
| Redundancy | 7.12 | 4.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8 | 298 | 0.2M Tris pH 8.0, 0.2M ammonium sulphate, 0.6M 1,6-hexane diol, 5mM mercaptoethanol, 4% glycerol, microbatch, temperature 298K |
