1ZYM

AMINO TERMINAL DOMAIN OF ENZYME I FROM ESCHERICHIA COLI

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-11-03
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	47.420, 75.780, 170.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.205
Rwork	0.205
R-free	0.30600
RMSD bond length	0.014
RMSD bond angle	24.200 *
Data reduction software	PROCESS
Data scaling software	SCALE
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.550
High resolution limit [Å]	2.500	2.500
Rmerge	0.097
Total number of observations	128042 *
Number of reflections	20342
Completeness [%]	92.0	66
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6-12 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	PEG4000	20-25 (%(w/v))
4	1	reservoir	cacodylate	100 (mM)
5	1	reservoir	potassium sulfate	50 (mM)