1ZY2

Crystal structure of the phosphorylated receiver domain of the transcription regulator NtrC1 from Aquifex aeolicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-23
Detector	ADSC QUANTUM 4
Wavelength(s)	1.100
Spacegroup name	P 31 2 1
Unit cell lengths	91.517, 91.517, 130.942
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.000 - 3.030
R-factor	0.2539
Rwork	0.251
R-free	0.28290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1NY5 RESIDUES 1-124 OF CHAIN A
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.300	3.190
High resolution limit [Å]	3.030	3.030
Number of reflections	12453
Completeness [%]	98.0	97.9
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	310	PEG-3350, AMMONIUM CHLORIDE, GLYCEROL, CITRIC ACID, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K