1ZXY
Anthranilate Phosphoribosyltransferase from Sulfolobus solfataricus in complex with PRPP and Magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.815 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 92.694, 65.806, 116.722 |
| Unit cell angles | 90.00, 107.80, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.560 |
| Rwork | 0.204 |
| R-free | 0.26000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1o17 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.394 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.670 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Number of reflections | 42220 | |
| <I/σ(I)> | 10.43 | 3.22 |
| Completeness [%] | 94.6 | 69.3 |
| Redundancy | 3.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 1500, MES , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
