1ZXQ

THE CRYSTAL STRUCTURE OF ICAM-2

Experimental procedure

Temperature [K]	113
Detector technology	AREA DETECTOR
Collection date	1995-04-20
Detector	SIEMENS
Spacegroup name	C 1 2 1
Unit cell lengths	69.000, 63.700, 76.200
Unit cell angles	90.00, 114.50, 90.00

Refinement procedure

Resolution	15.000 - 2.200
R-factor	0.226
Rwork	0.226
R-free	0.29500
Structure solution method	MIR
RMSD bond length	0.019
RMSD bond angle	2.200
Data reduction software	CCP4
Data scaling software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.440	0.141
Number of reflections	14277
<I/σ(I)>	10.5	4.2
Completeness [%]	93.9	68.8
Redundancy	3.1	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	4 *	CRYSTALS WERE BEST GROWN AT 4C USING THE HANGING-DROP VAPOR DIFFUSION METHOD, WITH A PROTEIN SOLUTION OF 15-17 MG/ML AND A CRYSTALLIZATION SOLUTION WITH 20% PEG 4000, 20 MM CACODYLATE BUFFER PH 6.4, AND 25 MM B-OCTYL-GLUCOPYRANOSIDE., vapor diffusion - hanging drop, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-17 (mg/ml)
2	1	reservoir	PEG4000	20 (%)
3	1	reservoir	cacodylate	20 (mM)
4	1	reservoir	beta-octyl glucopyranoside	25 (mM)