1ZXJ

Crystal structure of the hypthetical Mycoplasma protein, MPN555

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	132.370, 45.590, 153.930
Unit cell angles	90.00, 111.40, 90.00

Refinement procedure

Resolution	19.810 - 2.800
Rwork	0.248
R-free	0.32200
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHENIX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.760	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	38935
<I/σ(I)>	6.7
Completeness [%]	94.4	91.5
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	Bis/Tris, ammonium sulfate, pentaerythritol ethoxylate, zwittergent 3-14, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K