1ZX7
Molecular Recognition of RNA by Neomycin and a Restricted Neomycin Derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-10-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.650, 153.200, 32.740 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.150 |
| R-factor | 0.262 |
| Rwork | 0.251 |
| R-free | 0.29230 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.976 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.071 | 0.384 |
| Number of reflections | 16300 | |
| <I/σ(I)> | 23 | 3.3 |
| Completeness [%] | 93.5 | 72.2 |
| Redundancy | 17.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.8 | 298 | ammonium sulfate, magnesium chloride, cacodylate, pH 6.8, EVAPORATION, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulfate | ||
| 2 | 1 | 1 | magnesium chloride | ||
| 3 | 1 | 1 | cacodylate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | ammonium sulfate | ||
| 6 | 1 | 2 | magnesium chloride | ||
| 7 | 1 | 2 | cacodylate | ||
| 8 | 1 | 2 | H2O |
