1ZX6
High-resolution crystal structure of yeast Pin3 SH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.813 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.040, 27.360, 24.000 |
| Unit cell angles | 90.00, 102.87, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.18347 |
| Rwork | 0.182 |
| R-free | 0.22089 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1oot |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.451 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 7155 | |
| <I/σ(I)> | 7.9 | 2.6 |
| Completeness [%] | 94.2 | 96.8 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | VAPOR DIFFUSION, HANGING DROP, temperature 295K |
