1ZX5
The structure of a putative mannosephosphate isomerase from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2004-10-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 160.045, 160.045, 160.045 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.720 - 2.300 |
| R-factor | 0.16383 |
| Rwork | 0.162 |
| R-free | 0.19081 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.264 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.720 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 31599 | |
| <I/σ(I)> | 9.1 | 4.3 |
| Completeness [%] | 99.9 | 97.8 |
| Redundancy | 9.4 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 298 | PEG 5k MME, Na Acetate, ethylene glycol, sucrose, glycerol, pH 5.6, VAPOR DIFFUSION, temperature 298K |
